abstract：:The effects of 6-diazo-5-oxo-L-norleucine (DON), 2-deoxy-D-glucose (DOG), and tunicamycin (TM) on the replication of poliovirus (PV) and vesicular stomatitis virus (VSV) were examined. During a 48-hr replication period, TM, DON, and DOG inhibit VSV plaque formation in HEp-2 cells by 99.9%, 99.8%, and 99.9% respectivel...

journal_title：Journal of medical virology

authors： Goldstein G,Guskey LE

更新日期：1984-01-01 00:00:00

abstract：:The prevalence of delta agent among 120 Chinese patients with HBsAg-positive chronic active and hepatocellular carcinoma from Taiwan and Los Angeles were studied by serological assay for antibody to delta antigen and immunoperoxidase staining for delta antigen in the liver biopsies. Of 120 patients, only four with chr...

journal_title：Journal of medical virology

authors： Govindarajan S,Lee SD,Tong MJ,Tsai YT,Lo KJ

更新日期：1984-01-01 00:00:00

abstract：:We designed experiments to assess the effects of binding of the HSV-2 Fc receptor (FcR) to purified rabbit nonimmune IgG and purified Fc. Purified Fc (75 micrograms) or nonimmune IgG (100 micrograms) when bound to HSV-2 did not reduce infectivity but did protect the virions against thermal inactivation at 37 degrees C...

journal_title：Journal of medical virology

authors： Dowler KW,Veltri RW

更新日期：1984-01-01 00:00:00

abstract：:The competitive ELISA test was compared with competitive RIA, complement fixation, and indirect fluorescent antibody test for the detection of antibody to varicella-zoster virus (VZV). ELISA and RIA were the most sensitive tests, being tenfold as sensitive as indirect immunofluorescence and 20 times as sensitive as co...

journal_title：Journal of medical virology

authors： Wreghitt TG,Tedder RS,Nagington J,Ferns RB

更新日期：1984-01-01 00:00:00

abstract：:Enzyme immunoassay (EIA) for parainfluenza virus type 1, 2, and 3 antibodies was compared with the complement fixation test (CF) as a diagnostic method in 180 patients with respiratory symptoms. The CF test detected rises in parainfluenza virus antibodies in 30 cases, whereas EIA detected 47 rises. Patients with antib...

journal_title：Journal of medical virology

authors： Julkunen I

更新日期：1984-01-01 00:00:00

abstract：:Herpes simplex virus (HSV)-specific IgG and IgA antibody response in patients with HSV infection and in healthy adults was studied by the immunoperoxidase antibody-membrane antigen (IPAMA) technique. In all HSV infections in which specific IgM antibodies were detected by enzyme-linked immunosorbent assay (ELISA), a si...

journal_title：Journal of medical virology

authors： Hadar T,Sarov I

更新日期：1984-01-01 00:00:00

abstract：:Systemic cell-mediated responses to respiratory syncytial (RS) virus were detected, using a whole blood transformation assay, in 10 of 28 infants and children with RS virus infections during the period 1-14 days postadmission. Cell-mediated responses were unrelated to the age of the patient or the severity of illness....

journal_title：Journal of medical virology

authors： Scott R,Pullan CR,Scott M,McQuillin J

更新日期：1984-01-01 00:00:00

abstract：:Strains of human coronavirus (HCV) isolated between 1974 and 1976 have been studied in vitro and in volunteers. All strains caused colds in volunteers, and those cultivable in tissue culture (TC) produced significantly more coryza and less sore throat than strains growing only in organ culture (OC). The TC strains wer...

journal_title：Journal of medical virology

authors： Reed SE

更新日期：1984-01-01 00:00:00

abstract：:Thirty susceptible rhesus monkeys were inoculated with cell-free varicella-zoster virus strain OKA or strain KMcC. Both wild and attenuated strains were used. No clinical signs characteristic of human varicella were seen in any of the animals. Virus was not isolated from throat swabs, blood, or cerebrospinal fluid. An...

journal_title：Journal of medical virology

authors： Asano Y,Albrecht P,Behr DE,Neff BJ,Vickers JH,Rastogi SC

更新日期：1984-01-01 00:00:00

abstract：:Three monoclonal antibodies against herpes simplex virus type 2 have been tested for their suitability as reagents in reverse passive haemagglutination. Two of these antibodies with specificity for virus glycoprotein D, when linked to red blood cells, were able to capture antigens without being agglutinated, but addit...

journal_title：Journal of medical virology

authors： Cranage MP,McLean CS,Buckmaster EA,Minson AC,Wildy P,Coombs RR

更新日期：1983-01-01 00:00:00

abstract：:A possible new subgroup of human rotavirus was found by crossed immunoelectrophoresis and enzyme-linked immunosorbent assay. The epidemiology of the established subgroups 1 and 2 and this new variant was studied in two different communities. Of 398 rotavirus isolates from Malmö, Sweden, 26.8% were of type 1, 71.7% of ...

journal_title：Journal of medical virology

authors： Tufvesson B

更新日期：1983-01-01 00:00:00

abstract：:Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)-denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenic...

journal_title：Journal of medical virology

authors： Sanchez Y,Ionescu-Matiu I,Melnick JL,Dreesman GR

更新日期：1983-01-01 00:00:00

abstract：:Junin virus infection in guinea pigs is known to be similar to human Argentine hemorrhagic fever (AHF). The guinea pig was chosen as a model for transplacental transmission of Junin virus, as both guinea pig and man have a similar placental structure. Pregnant guinea pigs were infected with the pathogenic XJ strain of...

journal_title：Journal of medical virology

authors： Sangiorgio P,Weissenbacher MC

更新日期：1983-01-01 00:00:00

abstract：:A method for the absorption of false radioimmunoassay (RIA) IgM titres against herpes simplex virus (HSV) and cytomegalovirus (CMV) is presented. The serum specimens were absorbed by a mixture of protein A-Sepharose and protein A-Sepharose saturated with normal human gamma globulin (PAS/IgG). The detection of rheumato...

journal_title：Journal of medical virology

authors： Torfason EG,Diderholm H

更新日期：1982-01-01 00:00:00

abstract：:To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...

journal_title：Journal of medical virology

authors： Schmidt NJ

更新日期：1982-01-01 00:00:00

abstract：:After an 8-day-old child had died with clinical signs of septicemia, 6 other newborns fell ill. Virus was isolated from various sites from all the 6 children (28 isolations). The agent was identified by cross neutralization tests as an antigenic variant of Echovirus 11. The agent could not be isolated from mothers or ...

journal_title：Journal of medical virology

authors： Mertens T,Hager H,Eggers HJ

更新日期：1982-01-01 00:00:00

abstract：:Rotavirus was detected in the stools of five children stricken with sudden infant death syndrome (SIDS) over a three-week period. While none of the children had acute gastroenteritis, four of the five had acute upper respiratory infections. Rotavirus was also identified in tracheal aspirates from two of the infants. E...

journal_title：Journal of medical virology

authors： Yolken R,Murphy M

更新日期：1982-01-01 00:00:00

abstract：:A study was carried out to determine whether hepatitis A virus (HAV) can be detected in the stools of patients hospitalized for HAV infection. Acute phase samples of whole blood and stool, as well as completed questionnaires, were obtained from 31 patients hospitalized at any of 13 hospitals in the Phoenix metropolita...

journal_title：Journal of medical virology

authors： Carl M,Kantor RJ,Webster HM,Fields HA,Maynard JE

更新日期：1982-01-01 00:00:00

abstract：:Infectivity of certain enteric viruses including rotavirus is profoundly affected by proteolytic enzymes. To test whether cystic fibrosis patients, possessing chronically decreased levels of pancreatic enzymes, show altered susceptibility to gastroenteritis viruses, we examined sera from patients and controls for anti...

journal_title：Journal of medical virology

authors： Cukor G,Blacklow NR,Braverman LE

更新日期：1982-01-01 00:00:00

abstract：:Many of the small round human fecal viruses implicated in outbreaks of nonbacterial gastroenteritis have been collected together and examined under the electron microscope. Negatively stained preparations without the addition of antibody were used so that the surface morphology of the virus particles remained clearly ...

journal_title：Journal of medical virology

authors： Caul EO,Appleton H

更新日期：1982-01-01 00:00:00

abstract：:The presence of antibody to hepatitis A virus (anti-HAV) in 60% of procured owl monkeys (Aotus trivirgatus) held within the United States prompted a study of recently captured A trivirgatus in Panama. Only 2 of 145 newly captured monkeys, but all of 35 A trivirgatus held within a colony for over 100 days, were found t...

journal_title：Journal of medical virology

authors： Lemon SM,LeDuc JW,Binn LN,Escajadillo A,Ishak KG

更新日期：1982-01-01 00:00:00

abstract：:Seventeen out of 30 patients with chronic hepatitis type B with hepatitis B e antigen (HBeAg) in serum remained persistently positive for e antigen, while 13 seroconverted to antibody (anti-HBe) when followed over a period of one to five years. Initial levels of serum hepatitis B virus (HBV) markers, such as the hepat...

journal_title：Journal of medical virology

authors： Alberti A,Pontisso P,Realdi G

更新日期：1982-01-01 00:00:00

abstract：:In a study in Kenya of corneal lesions occurring in children with measles virus infection, conjunctival lesions of a characteristic appearance were seen concomitant with the rash. The lesions became visible after staining by Lissamine green or Rose Bengal. In order to investigate whether these lesions were virus-induc...

journal_title：Journal of medical virology

authors： Nommensen FE,Dekkers NW

更新日期：1981-01-01 00:00:00

abstract：:Using immunodiffusion, a major cross-reactivity had been previously demonstrated between hepatitis B(HBe/3 Ag) and the antigen reported in the serum on non A, non-B hepatitis patients, therefore redesignated NANBe Ag. By direct immunofluorescence a new antigen associated with, but distinct from, NANBe Ag has now been ...

journal_title：Journal of medical virology

authors： Trépo C,Vitvitski L,Hantz O

更新日期：1981-01-01 00:00:00

abstract：:The multiplication of HSV-1 and HSV-2 strains in the heart and the corresponding dorsal root ganglia (DRG) was examined in experimentally infected mice. Infectious HSV-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the DRG. Both the heart and DRG yielded infect...

journal_title：Journal of medical virology

authors： Grodums EI,Kuling PJ,Zbitnew A

更新日期：1981-01-01 00:00:00

abstract：:Immunoglobulin G (IgG) and M (IgM) antibodies against mumps virus were measured by an enzyme-linked immunosorbent assay (ELISA) in the serum and cerebrospinal fluid (CSF) specimens of patients with mumps meningitis. The CSF IgG antibodies correlated well with the respective antibody titers in serum. On the contrary, i...

journal_title：Journal of medical virology

authors： Ukkonen P,Granström ML,Räsänen J,Salonen EM,Penttinen K

更新日期：1981-01-01 00:00:00

abstract：:human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell culture-adapted HAV, and 50 days after i...

journal_title：Journal of medical virology

authors： Gauss-Müller V,Frösner GG,Deinhardt F

更新日期：1981-01-01 00:00:00

abstract：:During the 1976 Ebola virus outbreak in Sudan, the investigations team gained the impression that fewer haemorrhagic manifestations and few fatalities occurred during the later stages of the epidemic after the virus had undergone several generations in man. This impression was also noted in guinea pigs experimentally ...

journal_title：Journal of medical virology

authors： Bowen ET,Platt GS,Lloyd G,Raymond RT,Simpson DI

更新日期：1980-01-01 00:00:00

abstract：:Following widespread outbreaks of oyster-associated gastroenteritis in Australia during 1978 in which Norwalk virus was implicated as the causative agent, collaborative studies were undertaken between laboratories in Australia and the United States to confirm the etiology. Immune electron microscopy (IEM) techniques w...

journal_title：Journal of medical virology

authors： Grohmann GS,Greenberg HB,Welch BM,Murphy AM

更新日期：1980-01-01 00:00:00

abstract：:Nasal washings were collected from 27 normal adults during 38 naturally acquired colds. The washings were exhaustively tested using tissue cultures, organ cultures and electron microscopy. Washings yielding no identifiable agent were inoculated into human volunteers, and further specimens obtained from the latter were...

journal_title：Journal of medical virology

authors： Larson HE,Reed SE,Tyrrell DA

更新日期：1980-01-01 00:00:00

abstract：:An enzyme immunoassay utilizing a fluorogenic substrate (EIFA) was employed to compare humoral antibody responses to purified virion and dense body antigens in human cytomegalovirus (CMV) infections. Results of these antibody assays were also compared to those obtained by plaque reduction neutralization and complement...

journal_title：Journal of medical virology

authors： Forghani B,Schmidt NJ

更新日期：1980-01-01 00:00:00

abstract：:Hemagglutination inhibition (HI), single radial hemolysis (SRH) and enzyme-linked immuno sorbent assay (ELISA), performed with commercial antigen and reagents are described and were compared in the three distinct situations that require rubella antibody detection. Determination of immunity status was carried out on 15...

journal_title：Journal of medical virology

authors： Champsaur H,Dussaix E,Tournier P

更新日期：1980-01-01 00:00:00

abstract：:In eight patients with biopsy-confirmed chronic active hepatitis (CAH), hepatitis B e antigen (HBeAg) levels and DNA polymerase (DNA-P) activity were assayed three times a week for six weeks and once a week for another six weeks. HBeAg levels were rather constant, whereas DNA-P activity fluctuated. No correlation was ...

journal_title：Journal of medical virology

authors： Heijtink RA,van der Waart M,Weimar W,Schalm SW,Masurel N

更新日期：1980-01-01 00:00:00

abstract：:In October 1977 an outbreak of acute infectious diarrhea occurred in an infant home in the city of Sapporo, Japan. Of 34 residents aged two to 20 months, 26 (77%) suffered from diarrhea. In ten of these patients the diarrhea was accompanied by vomiting. Electron microscopic examinations revealed typical calicivirus pa...

journal_title：Journal of medical virology

authors： Chiba S,Sakuma Y,Kogasaka R,Akihara M,Horino K,Nakao T,Fukui S

更新日期：1979-01-01 00:00:00

abstract：:A sensitive radioimmunoassay technique was used to detect hepatitis B e antigen (HBeAg). A strong correlation was found between HBeAg positivity of the serum of hepatitis B surface antigen (HBsAg) carrier women in Taiwan and the subsequent development of surface antigenemia in their babies. All babies who became chron...

journal_title：Journal of medical virology

authors： Stevens CE,Neurath RA,Beasley RP,Szmuness W

更新日期：1979-01-01 00:00:00

abstract：:Serial determinations of titers of binding antibodies to single stranded DNA were performed over a period of three years in 43 type B hepatitis patients with persisting HBsAg who had either developed chronic hepatitis or were asymptomatic carriers. Patients with histopathological diagnosis of chronic active or chronic...

journal_title：Journal of medical virology

authors： Villarejos VM,Serra J,Visoná KA,Eduarte CE

更新日期：1979-01-01 00:00:00

abstract：:Herpes simplex virus can be quantitatively recovered from vaginal tampons suspended in tissue culture medium and stored over a wide range of temperatures. Because herpes simplex virus is stable for several days with refrigeration or after freezing, this method of culture of cervicovaginal virus lends itself to epidemi...

journal_title：Journal of medical virology

authors： Myers MG,Ekwo EE,Pitts AK

更新日期：1979-01-01 00:00:00

abstract：:Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomati...

journal_title：Journal of medical virology

authors： Yamada E,Ohori H,Ishida N

更新日期：1979-01-01 00:00:00

abstract：:A solid-phase radioimmunoassay (RIA) for the detection of hepatitis B e antigen (HBeAg) and antibody (anti-HBe) was developed. The RIA was approximately 1,000-fold more sensitive than rheophoresis for HBeAg, and approximately 6,000-fold more sensitive than rheophoresis for anti-HBe. Generally, less than one-fifth of h...

journal_title：Journal of medical virology

authors： Mushahwar IK,Overby LR,Frosner G,Deinhardt F,Ling CM

更新日期：1978-01-01 00:00:00

abstract：:A thirteen-year-old boy with bilateral orchitis was diagnosed as being infected by Coxsackie A9 virus by isolation of the virus from a throat swab and a fecal specimen. Serologic investigations revealed the development of transitory specific neutralizing IgM antibodies against the isotate and of persistent antibodies ...

journal_title：Journal of medical virology

authors： Willems WR,Hornig C,Bauer H,Klingmüller V

更新日期：1978-01-01 00:00:00